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Facility
BioDesign 2021; 9(4): 72-77
Published online December 30, 2021
https://doi.org/10.34184/kssb.2021.9.4.72
© Korean Society for Structural Biology
Integrated cryoEM imaging center at Seoul National University, Korea
Miji Jeon†, Jun Bae Park†, Hansol Lee, Gyuhee Kim, Eung Baek Kim, Mingyu Jung and Soung-Hun Roh*
School of Biological Science, Institute of Molecular Biology and Genetics, Seoul National University, Seoul 08826, Republic of Korea
Correspondence to: *shroh@snu.ac.kr
†These authors contributed equally to this work.
Abstract
The Center for Macromolecular and Cell Imaging (CMCI) is a core facility equipped with multiple cryo-capable electron microscopes including a 200 kV Thermofisher Glacios, 200 kV JEOL JEM2100Plus and 120 kV FEI TECNAI with direct electron detector technologies. The center also has auxiliary equipment for optimal specimen preparation, such as for protein purification, tissue culture, grid preparation and vitrification. The center offers to the scientific community the best practice of cutting-edge cryogenic electron microscopy (cryoEM) technology for specimen optimization, high resolution data collection and structure determination. The center also develops the strategy of an integrated cryoEM imaging platform to visualize molecular architectures from purified proteins to cells.
