Growing demands for the structure determination of biological macromolecules using cryogenic electron microscopy (cryo-EM) necessitate expansion of national research infrastructure. In particular, better accessibility to transmission electron microscopes (TEMs) is needed for sample screening and high-resolution data collection as well as for newly emerging cryo-electron tomography (cryo-ET) analysis. Here we introduce recently established Cryo-EM Research Center at Pusan National University. The center is equipped with high-end TEM for data collection, lower-end TEM for sample screening and focused ion beam-scanning electron microscope (FIB-SEM) for sample milling at cryogenic temperature. Ancillary devices for sample vitrification and computational resources are also available for routine cryo- EM workflow. The center is anticipated to contribute to the development of structural biology field in Korea.