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Crystallization
BioDesign 2022; 10(2): 34-38
Published online June 30, 2022
https://doi.org/10.34184/kssb.2022.10.2.34
© Korean Society for Structural Biology
Pyridoxal phosphate homeostasis protein FnYggS from Fusobacterium nucleatum: purification, crystallization, and X-ray crystallographic analysis
Shanru He1,2, Chenyuan Yuan1,2, Xue Bai1,2, Tingting Bu1,2, Jie Zhang1,2, Lulu Wang1,2, Chunshan Quan1,2 and Yongbin Xu1,2,*
1Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian 116600, Liaoning, China
2Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, College of Life Science, Dalian Minzu University, Dalian 116600, Liaoning, China
Correspondence to: *yongbinxu@dlnu.edu.cn
Abstract
Anaerobic gram-negative bacterium Fusobacterium nucleatum (F. nucleatum) has long been found to cause opportunistic infections and has recently been implicated in colorectal cancer. In F. nucleatum, YggS (FnYggS) is an important part of bacterial cell walls and belongs to the COG0325 gene family. In this study, YggS from FnYggS was successfully expressed and purified using Ni-NTA affinity and gel-filtration chromatography. The protein crystal was obtained and diffracted to a resolution of 2.08 Å. The preliminary crystallographic analysis suggested that FnYggS crystal belongs to the monoclinic space group P21 with a = 37.93 Å, b = 146.38 Å, and c = 74.13 Å, α = γ = 90.00° and β = 93.36°. The asymmetric unit contained approximately three monomer of FnYggS, giving a crystal volume per mass (VM) of 2.64 Å3 Da–1 and a solvent content of 53.50%.
