pISSN 2288-6982
eISSN 2288-7105




BioDesign 2022; 10(4): 79-85

Published online December 30, 2022

© Korean Society for Structural Biology

Cryo-Electron Microscopy Research Center at Pusan National University

Bokyeong Park1,†, Sujin Lee1,†, Yeontae Jang2,†, Seu-Na Lee3, Seungmi Kim2, Jae-Sung Woo3, Kyung Lib Jang4 and Jaekyung Hyun1,2,*

1Core Research Facility, Pusan National University, Yangsan 50612, Republic of Korea
2Department of Convergence Medicine, School of Medicine, Pusan National University, Yangsan 50612, Republic of Korea
3Department of Life Sciences, Korea University, Seoul 02841, Republic of Korea
4Department of Microbiology, College of Natural Science, Pusan National University, Busan 46241, Republic of Korea

Correspondence to: *
These authors contributed equally to this work.

Received: December 7, 2022; Accepted: December 20, 2022


Growing demands for the structure determination of biological macromolecules using cryogenic electron microscopy (cryo-EM) necessitate expansion of national research infrastructure. In particular, better accessibility to transmission electron microscopes (TEMs) is needed for sample screening and high-resolution data collection as well as for newly emerging cryo-electron tomography (cryo-ET) analysis. Here we introduce recently established Cryo-EM Research Center at Pusan National University. The center is equipped with high-end TEM for data collection, lower-end TEM for sample screening and focused ion beam-scanning electron microscope (FIB-SEM) for sample milling at cryogenic temperature. Ancillary devices for sample vitrification and computational resources are also available for routine cryo- EM workflow. The center is anticipated to contribute to the development of structural biology field in Korea.